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Image Search Results
Journal: Communications Biology
Article Title: Dynamic full-field optical coherence tomography allows live imaging of retinal pigment epithelium stress model
doi: 10.1038/s42003-022-03479-6
Figure Lengend Snippet: a Drawing of our custom-built Full-Field OCT system. BS beam-splitter, HPF high-pass filter, PZT piezo-electric translation, TS translation stage (for the sample). b Schematic of the RPE cell layer before and after the scratch assay (from left to right). c Recombined three channels of HSV computation and the three different channels in lower left. The colorbar represents the frequency variations of the sample (Hue channel). d – g Results of the analysis of a closing scratch assay on a primary porcine RPE cell culture (ppRPE). d Beginning and end (i.e., closing) of the imaging of the scratch assay. The dotted white square corresponds to the area used for calculations. e , f Plots of the evolution of scratch width and wound closure over the acquisition, calculated with SAVE Profiler. g Optical flow calculations showing velocity and motion direction. h – k Results of the analysis of a closing scratch assay on a hiRPE cell culture. h Beginning and end (i.e., closing) of the imaging of the scratch assay. i , j Plots of the evolution of scratch width and wound closure over the acquisition, calculated with SAVE Profiler. k Optical flow calculations. (Statistics: see Supplementary Table (scale-bar: 50 μm).
Article Snippet: For this purpose, custom software was developed in
Techniques: Wound Healing Assay, Cell Culture, Imaging
Journal: Communications Biology
Article Title: Dynamic full-field optical coherence tomography allows live imaging of retinal pigment epithelium stress model
doi: 10.1038/s42003-022-03479-6
Figure Lengend Snippet: a Beginning and end of the imaging of a scratch assay on ppRPE cell culture. b , c Evolution of scratch width and wound closure calculated with our programme and d optical flow calculations. e Beginning and end of the imaging of a scratch assay on hiRPE cell culture. f , g Evolution of the scratch width and wound closure, calculated with the SAVE profiler and ( h ) optical flow calculations. (scale-bar: 50 μm) (Statistics: see Supplementary Table ).
Article Snippet: For this purpose, custom software was developed in
Techniques: Imaging, Wound Healing Assay, Cell Culture